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Western blot analysis of lysed extracts from 293 cells using MEK1/MEK2 (Ab-217/221).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MEK1/MEK2 (Ab-217/221).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK1/MEK2(Ab-217/221).
Western blot analysis of lysed extracts from 293 cells using MEK1/MEK2 (Ab-217/221).

MEK1 / MEK2 (Ab 217 / 221) Antibody

CATALOG NUMBER: 79-360

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MEK1/MEK2 (Ab-217/221) antibody was raised against a peptide sequence around aa.215~219/219~223 (I-D-S-M-A) derived from Human MEK1/MEK2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total MEK1/MEK2 protein.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K1 MAP2K2
Additional Names:
CFC3, MEK1, MKK1, MAPKK1, PRKMK1, GLUR2, ERK activator kinase 1/ERK activator kinase2, MAP kinase kinase 1/MAP kinase kinase 2, MAP2K1/MAP2K1, MAPK/ERK kinase 1/MAPK/ERK kinase 2, MAPKK 1/MAPKK 1
Protein Accession Number:
NP_002746.1;NP_109587.1
Protein Gi Number:
5579478 / 13489054
Ncbi Gene Id Number:
5604;5605
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Background Reference 1:
Shen R, et al. (2002) Mol Cell Biol; 22 (10): 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4): 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1): 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11): 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5): 2304-2310

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-360

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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