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Western blot analysis of lysed extracts from HepG2 cells untreated or treated with PMA using MEK1 (Phospho-Thr291).
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with PMA using MEK1 (Phospho-Thr291).

MEK1 (phospho Thr291) Antibody

CATALOG NUMBER: 79-495

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MEK1 (Phospho-Thr291) antibody was raised against a peptide sequence around phosphorylation site of threonine 291 (P-R-T (p) -P-G derived from Human MEK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K1
Additional Names:
CFC3, MEK1, MKK1, MAPKK1, PRKMK1, TYH, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1
Protein Accession Number:
NP_002746.1
Protein Gi Number:
5579478
Ncbi Gene Id Number:
5604
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Background Reference 1:
Kevin D. Burroughs, et, al. (2003) Mol. Cancer Res ; 1: 312. Michael J. Piatelli, et, al. (2002) J. Biol. Chem ; 277: 12144 - 12150. Margaret M. Morgan, et, al. (2001) J. Immunol ; 167: 5708. Herbert Schramek, et, al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-495

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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