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Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr386.

MEK1 (phospho Thr386) Antibody

CATALOG NUMBER: XPS-1017

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
MEK 1 (Thr386) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr386 of human MEK 1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
MEK1 antibody is specific for the 45k MEK 1 phosphorylated at Thr386.
Predicted Molecular Weight:
45
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
MAP2K1
Protein Accession Number:
Q02750
Protein Gi Number:
400274
Ncbi Gene Id Number:
5604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.
Background Reference 1:
Park, S.H., Zarrinpar, A. and Lim, W.A., "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms," Science 299 (2003) 1061 - 1064.
Background Reference 2:
Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.
Background Reference 3:
Mansour, S.J., Resing, K.A., Candi, J.M., Hermann, A.S., Gloor, J.W., Herskind, K.R., Wartmann, M., Davis, R.J., and Ahn, N.G., "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." J. Biochem. (Tokyo) 116 (1994) 304-14.
Background Reference 4:
Ahn, N.G., "The MAP kinase cascade. Discovery of a new signal transduction pathway," Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1017

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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