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Western blot analysis of MAP2K4 using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MAP2K4 using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2).

MEK4 Antibody

CATALOG NUMBER: 63-286

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse
Immunogen:
This MEK4(MAP2K4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from human MEK4(MAP2K4).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP2K4
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1
Protein Accession Number:
P45985
Protein Gi Number:
1170596
Ncbi Gene Id Number:
6416
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Background Reference 1:
Robinson,V.L.,Mol. Cancer Res.(3), 501-508 (2008)
Background Reference 2:
Zhang,H.,J. Biol. Chem. 282 (20), 14788-14796 (2007)
Background Reference 3:
Salmeron,A.,EMBO J. 15 (4), 817-826 (1996)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-286

List Size:
400 ul

List Price:

$495.00

Datasheet
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