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Western blot analysis of lysed extracts from Rat and Mouse brain tissue using MEK5 (phospho-Ser311/ Thr315).
Western blot analysis of lysed extracts from Rat and Mouse brain tissue using MEK5 (phospho-Ser311/ Thr315).

MEK5 (phospho Ser311 / Thr315) Antibody

CATALOG NUMBER: 79-516

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MEK5 (phospho-Ser311/ Thr315) antibody was raised against a peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S (p) I-A-K-T (p) -Y-V) derived from Rat MEK5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.
Predicted Molecular Weight:
46 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
Map2k5
Additional Names:
Mek5, CDC2, CDC28A, CDKN1, P34CDC2, Map2k5
Protein Accession Number:
NP_001029159.1
Protein Gi Number:
77157802
Ncbi Gene Id Number:
29568
Background:
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Background Reference 1:
English J. M., Vanderbilt C. A., Xu S., Marcus S., Cobb M. H. J. Biol. Chem. 270:28897-28902 (1995)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-516

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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