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Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.
Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.
SDS-PAGE Analysis of Purified, BSA-Free Melan-A Antibody (clone M2-9E3). Confirmation of Integrity and Purity of the Antibody.
Formalin/paraffin human melanoma stained with MART-1 / Melan-A antibody (M2-9E3). Note cytoplasmic staining of cells.

Melan-A Antibody [M2-9E3]

CATALOG NUMBER: 33-131

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human Melan-A protein was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, IP, WB
Application Note:
ELISA: order BSA-free format for coating
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IP: 0.5-1ug/500ug protein lysate
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MLANA
Additional Names:
MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
Ncbi Gene Id Number:
2315
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-131

List Size:
100 ug

List Price:

$626.00

Datasheet
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