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IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Melan-A / MART-1 Antibody

CATALOG NUMBER: 33-646

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1ug/10^6 cells
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT
The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.
Purification:
Protein A affinity chromatography
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MLANA
Additional Names:
MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1
Ncbi Gene Id Number:
2315
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-646

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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