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45-882 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.
Wild-type C57bl6 derived melanocytes were stained with 45-882 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.
45-882 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.

Melanophilin Antibody

CATALOG NUMBER: 45-882

Clonality:
Polyclonal
Tested Applications:
ELISA, GST-PD, ICC, IF, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-ARHIFAKPVMAQQP
Conjugate:
Unconjugated
Tested Applications:
ELISA, GST-PD, ICC, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on Mouse: Sanzà Pet al (PMID: 30898842), Singh RK et al (PMID: 23281710), Figueiredo AC et al (PMID: 18559336) and Hume AN et al (PMID: 16914517).Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. PMID:18559336.
GST-pull down assay: See publication Figueiredo AC et al., J Biol Chem. 2008 Aug 22;283(34):23209-16. (PMID:18559336).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Mlph
Additional Names:
2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac-2a, D1Wsu84e, l(1)-3Rk, 5031433I09Rik, leaden, AW228792, OTTMUSP00000025451
Protein Accession Number:
NP_443748.2
Protein Gi Number:
87080831
Ncbi Gene Id Number:
171531(mouse);316620(rat);
Background Reference 1:
Matesic LE, Yip R, Reuss AE, Swing DA, O'Sullivan TN, Fletcher CF, Copeland NG, Jenkins NA. Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice. Proc Natl Acad Sci USA. 2001 Aug 28;98(18):10238-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-882

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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