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<b> Immunofluorescence staining of fixed HeLa cells with anti-membrane type 1 metalloprotease antibody LEM-2/15 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of LEM-2/15 (24-349) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing nuclear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-349- DAPI- merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-MT1-MMP (Membrane type 1 metalloprotease) antibody LEM-2/15 (</b> A549 (A)- HepG2 (B) and HeLa (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of LEM-2/15 (24-349) at 0.3 ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unprocessed MT1-MMP is 65.9 kDa. A signal peptide and propeptide are cleaved from MT1-MMP during post-translational processing. The processed form of MT1-MMP may be seen in all samples- and the unprocessed form in lanes B and C. 24-349 successfully detected MT1-MMP in A549- HepG2 and HeLa cell lysates.
Immunofluorescence staining of fixed HeLa cells with anti-membrane type 1 metalloprotease antibody LEM-2/15 ( Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of LEM-2/15 (24-349) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing nuclear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-349- DAPI- merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Membrane type 1 metalloprotease Antibody [LEM-2/15], Mouse IgG1

CATALOG NUMBER: 24-349

Clonality:
Recombinant Monoclonal
Tested Applications:
Blocking, ELISA
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Mouse
Original Species Name:
Mouse
Immunogen:
The cyclic peptide possessing the sequence of residues 218-233 of the V-B-loop of MT1-MMP.
Conjugate:
Unconjugated
Tested Applications:
Blocking, ELISA
Clonality:
Recombinant Monoclonal
Isotype:
IgG1 kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Additional Names:
MT1-MMP
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-349

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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