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45-883 (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-883 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-883 (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MEPCE Antibody

CATALOG NUMBER: 45-883

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: KQQRKFQYGNYCK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human for this particular batch.Immunohistochemistry:In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10ug/ml.
Positive Control 1:
Cat. No. 1476 - Rat Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MEPCE
Additional Names:
MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog
Protein Accession Number:
NP_062552.2
Protein Gi Number:
47271406
Ncbi Gene Id Number:
56257
Background Reference 1:
Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Mol Cell. 2007 Jul 20;27(2):262-74.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-883

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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