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45-884 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-884 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining
45-884 (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MEPI Antibody

CATALOG NUMBER: 45-884

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KGDWKQKFRKEDTQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1).
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SERPINI2
Additional Names:
SERPINI2, MEPI, PI14, PANCPIN, TSA2004, SERPIN12, serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2, serpin peptidase inhibitor, clade I (pancpin), member 2, myoepithelium-derived serine protease inhibitor, protease inhibitor 14, serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2
Protein Accession Number:
NP_006208.1,NP_001012303.1
Protein Gi Number:
5453886
Ncbi Gene Id Number:
5276
Background Reference 1:
Ozaki K, Nagata M, Suzuki M, Fujiwara T, Miyoshi Y, Ishikawa O, Ohigashi H, Imaoka S, Takahashi E, Nakamura Y. Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells. Genes Chromosomes Cancer. 1998 Jul;22(3):179-85.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-884

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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