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Recombinant MERS-CoV Spike RBD Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD26 (Catalog: 85-366) at 2μg/mL (100μL/well) can bind MERS-CoV Spike RBD with a linear range of 0.5-139.82ng/mL.
Recombinant MERS-CoV Spike RBD Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

MERS-CoV Spike RBD Recombinant Protein (85-371)

CATALOG NUMBER: 85-371

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
MERS-CoV
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu367-Tyr606
Fusion Tag:
C-mFc
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
51.84 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human CD26 at 2μg/mL (100μL/well) can bind MERS-CoV Spike RBD with a linear range of 0.5-139.82ng/mL.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
MERS-CoV Spike RBD
Protein Accession Number:
YP_009047204.1
Ncbi Gene Id Number:
14254594
Background:
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-371

List Size:
0.1 mg

List Price:

$448.00

Datasheet
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