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42-158 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-158 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MEST Antibody

CATALOG NUMBER: 42-158

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QELLYRYKQNRSGR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in Mouse and Rat Testis lysates (calculated MW of 37.6kDa according to Human NP_803490.1 and 38.8kDa according to Rat NP_001009617.1). Recommended concentration: 0.2-0.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.
Positive Control 1:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MEST
Additional Names:
MEST, mesoderm specific transcript homolog (mouse), DKFZp686L18234, MGC111102, MGC8703, PEG1, mesoderm specific transcript, paternally expressed gene 1
Protein Accession Number:
NP_002393.2,NP_803490.1
Protein Gi Number:
29294639
Ncbi Gene Id Number:
4232
Background Reference 1:
Li T, Vu TH, Lee KO, Yang Y, Nguyen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR, An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. J. Biol. Chem. 2002 Apr 277 (16): 13518-27.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-158

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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