Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot of 10µg of HEK 293 cells expressing mGluR1a and mGluR5 showing specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a.
Western blot of 10µg of HEK 293 cells expressing mGluR1a and mGluR5 showing specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a.

Metabotropic Glutamate Receptor 1a Antibody

CATALOG NUMBER: 50-101

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
mGluR 1a polyclonal antibody was raised against a peptide from the C-terminal region of mGluR1a.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Metabotropic Glutamate Receptor 1a antibody allows for strong and specific immunolabeling of ~125k mGluR1a and the ~250k mGluR1a dimer in Western blots of rat brain at antibody dilution of 1:1000. Immunolabeling blocked by preadsorption of antibody with the C-terminal peptide immunogen. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). Rabbit anti-mGluR recognizes mGluR1a in rat brain. When internally tested under ideal conditions the working dilutions were 1:500 for IHC and 1:1000 for DB and WB.
Predicted Molecular Weight:
125/250
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
Metabotropic Glutamate Receptor 1a antibody can be stored at -20°C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20°C. This product is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Grm1
Protein Accession Number:
P23385
Protein Gi Number:
121300
Ncbi Gene Id Number:
24414
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase.
Background Reference 1:
Kim, S.J., Kim, Y,S, Yuan, J.P., Petralia, R.S., Worley, P.F. and Linden, D.J., "Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1", Nature (London) 426 (2003) 285 - 291.
Background Reference 2:
Rammes, G., Palmer, M., Eder, M., Dodt, H.U., Zieglgansberger, W. and Collingridge, G.L., "Activation of mGlu receptors induces LTD without affecting postsynaptic sensitivity of CA1 neurons in rat hippocampal slices," J. Physiol 546 (2003) 455 - 460.
Background Reference 3:
Ferré, S., Karcz-Kubicha, M., Hope, B.T., Popoli, P., Burgueño, J., Gutiérrez, M.S., Casadó, V., Fuxe, K., Goldberg, S.R., Lluis, C. Franco, R. and Ciruela, F., "Synergistic interaction between adenosine A2A and glutamate mGlu5 receptors: Implications for striatal neuronal function," Proc. Natl. Acad. Sci. USA 99 (2002) 11,940-11,945.
Background Reference 4:
Huber, K.M., Kayser, M.S., and Bear, M.F., "Role for rapid dendritic protein synthesis in hippocampal mGluR-dependent long-term depression," Science 288 (2000) 1254 - 1256.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-101

List Size:
0.1 mL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369