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43-154 (0.2ug/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35ug protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Detected by chemiluminescence.
43-154 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-154 (0.2ug/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35ug protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Detected by chemiluminescence.

MEX3C Antibody

CATALOG NUMBER: 43-154

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TPRLSPTFPESIEH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60+36kDa bands observed in Mouse Testis lysates, and these bands reduced after knock-down by gene trap (calculated MW of 69.4kDa according to NP_057710.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Predicted Molecular Weight:
Approx 60 + 36kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MEX3C
Additional Names:
MEX3C, mex-3 homolog C (C. elegans), FLJ38871, MEX-3C, RKHD2, RNF194, OTTHUMP00000163545, RING finger and KH domain-containing protein 2, RING finger protein 194, RNA-binding protein MEX3C, ring finger and KH domain containing 2
Protein Accession Number:
NP_057710.3
Protein Gi Number:
148229134
Ncbi Gene Id Number:
51320
Background Reference 1:
Buchet-Poyau K, Courchet J, Le Hir H, Séraphin B, Scoazec JY, Duret L, Domon-Dell C, Freund JN, Billaud M.Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA binding proteins differentially localized to processing bodies.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-154

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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