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Western blot analysis of MFN1 in A431 cell lysate with MFN1 antibody at 1 μg/ml.
Immunocytochemistry of MFN1 in A431 cells with MFN1 antibody at 5 μg/mL.
Western blot analysis of MFN1 in A431 cell lysate with MFN1 antibody at 1 μg/ml.
Trial Size

MFN1 Antibody

CATALOG NUMBER: 7861

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MFN1 antibody was raised against a 17 amino acid peptide near the amino terminus of human MFN1.
The immunogen is located within amino acids 20 - 70 of MFN1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
MFN1 antibody can be used for detection of MFN1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
MFN1 antibody is human, mouse and rat reactive. MFN1 antibody is predicted to not cross-react with MFN2.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 82 kDa
Observed: 83 kDa
Purification:
MFN1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MFN1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MFN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MFN1
Additional Names:
MFN1 Antibody: hfzo1, hfzo2, Mitofusin-1, Fzo homolog
Protein Accession Number:
NP_284941
Protein Gi Number:
45269137
Ncbi Gene Id Number:
55669
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN1 and MFN2 form homotypic and heterotypic complexes and coordinately regulate mitochondrial fusion and are essential for embryonic development (3). When ectopically expressed, MFN1 inhibits the apoptosis-associated amino-terminal conformation change in the apoptotic protein Bax but not its mitochondrial translocation, indicating that MFN1 is involved in the regulating the activation of Bax on the outer mitochondrial membrane (4).
Background Reference 1:
Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
Background Reference 2:
Ishihara N, Eura Y, and Mihara K. Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity. J. Cell Sci. 2004; 117:6535-46.
Background Reference 3:
Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
Background Reference 4:
Ryu SW, Choi K, Park JH, et al. Mitofusin 1 inhibits an apoptosis-associated amino-terminal conformational change in Bax, but not its mitochondrial translocation, in a GTPase-dependent manner. Cancer Lett. 2012; 323:62-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7861

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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