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Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MGAT1 antibody (22-178) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MGAT1 antibody (22-178) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MGAT1 Antibody

CATALOG NUMBER: 22-178

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse liver
Positive Control 2:
Mouse kidney
Positive Control 3:
Mouse heart
Positive Control 4:
Rat liver
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MGAT1
Additional Names:
MGAT1, OTTHUMP00000161546, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase, MGAT, mannosyl (alpha-1, 3-)-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase, GNT-I, GNT-1, GLYT1, GLCT1, GLCNAC-TI, GGNT1
Ncbi Gene Id Number:
4245
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-178

List Size:
100 uL

List Price:

$436.00

Datasheet
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