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Western blot analysis of extracts of various cell lines, using MGEA5 antibody (23-776) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using MGEA5 antibody (23-776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

MGEA5 Antibody

CATALOG NUMBER: 23-776

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
A375
Positive Control 3:
BxPC-3
Positive Control 4:
HeLa
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MGEA5
Additional Names:
MEA5, MGEA5, NCOAT, protein O-GlcNAcase, N-acetyl-beta-D-glucosaminidase, N-acetyl-beta-glucosaminidase, beta-N-acetylglucosaminidase, beta-N-acetylhexosaminidase, beta-hexosaminidase, bifunctional protein NCOAT, hyaluronidase in meningioma, meningioma expressed antigen 5 (hyaluronidase), meningioma-expressed antigen 5, nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Ncbi Gene Id Number:
10724
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-776

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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