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Western blot analysis of extracts of various cell lines, using MGP antibody (19-662) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunohistochemistry of paraffin-embedded rat brain using MGP antibody (19-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MGP antibody (19-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using MGP antibody (19-662) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MGP antibody (19-662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

MGP Antibody

CATALOG NUMBER: 19-662

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-103 of human MGP (NP_000891.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
U-87MG
Positive Control 3:
A375
Positive Control 4:
BT-474
Positive Control 5:
3T3L1
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 12kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MGP
Additional Names:
MGP, GIG36, MGLAP, NTI
Ncbi Gene Id Number:
4256
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-662

List Size:
100 uL

List Price:

$436.00

Datasheet
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