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Western blot analysis in CEM cell line lysates (35ug/lane).
MGP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

MGP Antibody

CATALOG NUMBER: 56-137

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MGP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human MGP.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
12 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MGP
Additional Names:
Matrix Gla protein, MGP, Cell growth-inhibiting gene 36 protein, MGP, MGLAP
Protein Accession Number:
P08493
Protein Gi Number:
118572668
Ncbi Gene Id Number:
4256
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is secreted and likelyacts as an inhibitor of bone formation. The encoded protein isfound in the organic matrix of bone and cartilage. Defects in thisgene are a cause of Keutel syndrome (KS). Two transcript variantsencoding different isoforms have been found for this gene.
Background Reference 1:
Romero, R., et al. Am. J. Obstet. Gynecol. (2010) In press :
Background Reference 2:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 3:
Parker, B.D., et al. Ann. Intern. Med. 152(10):640-648(2010)
Background Reference 4:
Romero, R., et al. Am. J. Obstet. Gynecol. 202 (5), 431 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-137

List Size:
400 ul

List Price:

$495.00

Datasheet
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