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Western Blot at 1:4000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:4000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

MGST1 Antibody

CATALOG NUMBER: 61-481

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This MGST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-71 amino acids from the Central region of human MGST1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:4000
Predicted Molecular Weight:
18 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MGST1
Additional Names:
Microsomal glutathione S-transferase 1, Microsomal GST-1, Microsomal GST-I, MGST1, GST12, MGST
Protein Accession Number:
P10620
Protein Gi Number:
121740
Ncbi Gene Id Number:
4257
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds.This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress.
Background Reference 1:
Zhang,H., IntColorectal Dis 22 (10), 1185-1194 (2007)
Background Reference 2:
Prall,W.C., Mech. Ageing Dev. 128 (9), 503-510 (2007)
Background Reference 3:
Haines,J.L.,Invest. Ophthalmol. Vis. Sci. 47 (1), 329-335 (2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-481

List Size:
400 ul

List Price:

$495.00

Datasheet
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