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Western blot analysis of MGST2 Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with MGST2 Antibody ,which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MGST2 Antibody in HepG2 cell line lysates (35ug/lane)

MGST2 Antibody

CATALOG NUMBER: 63-683

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MGST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-147 amino acids from the C-terminal region of human MGST2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MGST2
Additional Names:
Microsomal glutathione S-transferase 2, Microsomal GST-2, Microsomal GST-II, MGST2, GST2
Protein Accession Number:
Q99735
Protein Gi Number:
2842764
Ncbi Gene Id Number:
4258
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, several of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. MGST2 is a protein which catalyzes the conjugation of leukotriene A4 and reduced glutathione to produce leukotriene C4.
Background Reference 1:
Jakobsson,P.J., et.al., Am. J. Respir. Crit. Care Med. 161 (2 PT 2), S20-S24 (2000)
Background Reference 2:
Sjostrom,M., et.al., Eur. J. Biochem. 268 (9), 2578-2586 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-683

List Size:
400 ul

List Price:

$495.00

Datasheet
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