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<b> Immunofluorescence staining of mouse splenocytes using anti-MHC I antibody (R1-9.6. </b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of R1-9.6 (24-542) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-542- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Immunofluorescence staining of mouse splenocytes using anti-MHC I antibody (R1-9.6. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of R1-9.6 (24-542) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-542- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

MHC I Antibody [R1-9.6], Rabbit IgG

CATALOG NUMBER: 24-542

Clonality:
Recombinant Monoclonal
Tested Applications:
IF, IP, radioimmunoassay
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
Conjugate:
Unconjugated
Tested Applications:
IF, IP, radioimmunoassay
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Additional Names:
R1.9.6, H-2, MHC-I, MHC Class I
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-542

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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