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To detect hMIA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIA (38-167) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.
To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1.0 ug/ml - 2.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary.
To detect hMIA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIA (38-167) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.

MIA Antibody

CATALOG NUMBER: 38-166

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA. Human MIA specific antibody was purified by affinity chromatography employing immobilized hMIA matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hMIA by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.
Sandwich
To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human MIA as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.
Western Blot:
To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MIA antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
MIA
Additional Names:
CD-RAPMelanoma-derived growth regulatory protein, Melanoma inhibitory activity protein,
Protein Accession Number:
Q16674
Protein Gi Number:
2498559
Ncbi Gene Id Number:
8190
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-166

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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