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43-213 (1ug/ml) staining of Human Glioma lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-213 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-213 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-213 (1ug/ml) staining of Human Glioma lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MIA / CD-RAP Antibody

CATALOG NUMBER: 43-213

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: PKLADRKLCADQE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 14kDa band observed in Human Glioma lysates (calculated MW of 14.5kDa according to NP_006524.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Skeletal Muscle. Recommended concentration: 5ug/ml.
Specificity:
The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1,
Predicted Molecular Weight:
Approx 14kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MIA / CD-RAP
Additional Names:
MIA, melanoma inhibitory activity, CD-RAP, melanoma-derived growth regulatory protein
Protein Accession Number:
NP_006524.1
Protein Gi Number:
2498559
Ncbi Gene Id Number:
8190
Background Reference 1:
Hofmann MA, Schicke B, Fritsch A, Biesold S, Gussmann F, Küchler I, Voit C, Trefzer U.Impact of lymph node metastases on serum level of melanoma inhibitory activity in stage III melanoma patients.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-213

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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