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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
MIA40 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

MIA40 Antibody

CATALOG NUMBER: 55-895

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MIA40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the C-terminal region of human MIA40.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHCHD4
Additional Names:
Mitochondrial intermembrane space import and assembly protein 40, Coiled-coil-helix-coiled-coil-helix domain-containing protein 4, CHCHD4, MIA40
Protein Accession Number:
Q8N4Q1
Protein Gi Number:
62510498
Ncbi Gene Id Number:
131474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CHCHD4, a component of human mitochondria, belongs to aprotein family whose members share 6 highly conserved cysteineresidues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus(Hofmann et al., 2005 [PubMed 16185709]).
Background Reference 1:
Daithankar, V.N., et al. Biochemistry 48(22):4828-4837(2009)
Background Reference 2:
Chacinska, A., et al. J. Biol. Chem. 283(44):29723-29729(2008)
Background Reference 3:
Terziyska, N., et al. FEBS Lett. 581(6):1098-1102(2007)
Background Reference 4:
Hofmann, S., et al. J. Mol. Biol. 353(3):517-528(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-895

List Size:
400 ul

List Price:

$495.00

Datasheet
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