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Western blot analysis of extracts of various cell lines, using MIB2 antibody (18-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using MIB2 antibody (18-124) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

MIB2 Antibody

CATALOG NUMBER: 18-124

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-580 of human MIB2 (NP_001164158.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HeLa
Positive Control 2:
BxPC-3
Positive Control 3:
U-87MG
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse testis
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MIB2
Additional Names:
ZZANK1, ZZZ5, E3 ubiquitin-protein ligase MIB2, RING-type E3 ubiquitin transferase MIB2, mind bomb homolog 2, mindbomb homolog 2, novel zinc finger protein, novelzin, putative NF-kappa-B-activating protein 002N, skeletrophin, zinc finger ZZ type with ankyrin repeat domain protein 1, zinc finger, ZZ type with ankyrin repeat domain 1
Ncbi Gene Id Number:
142678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-124

List Size:
100 uL

List Price:

$436.00

Datasheet
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