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45-886 (1ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-886 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-886 (1ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MIC1 Antibody

CATALOG NUMBER: 45-886

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DKLNHEYKKYLDAEQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Breast lysates (calculated MW of 75.0kDa according to NP_037458.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Small Intestine shows textured cytoplasm staining in of the enterocytes. Recommended concentration, 5-2=10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
C18orf8
Additional Names:
C18orf8, chromosome 18 open reading frame 8 , FLJ23453, HsT2591, MIC1 , colon cancer associated protein Mic1, colon cancer-associated protein Mic1
Protein Accession Number:
NP_037458.3
Protein Gi Number:
89242141
Ncbi Gene Id Number:
29919
Background Reference 1:
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M Global, in vivo, and site-specific phosphorylation dynamics in signalingnetworks. Cell. 2006 Nov 3;127(3):635-48.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-886

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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