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Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of MICA in human colon tissue with MICA antibody at 10 μg/mL.
Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 μg/mL.
Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

MICA Antibody

CATALOG NUMBER: 4261

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA.
The immunogen is located within the last 50 amino acids of MICA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 42 kDa
Observed: 46 kDa
Purification:
MICA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MICA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MICA antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MICA
Additional Names:
MICA Antibody: PERB11.1, PERB11.1, MHC class I polypeptide-related sequence A, MIC-A
Protein Accession Number:
NP_000238
Protein Gi Number:
4557751
Ncbi Gene Id Number:
4276
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MICA Antibody: Major histocompatibility complex (MHC) class I proteins are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic T cells. A related family, termed the MHC class I chain-related (MIC) proteins are recognized by NKG2D, a receptor on NK and T cells, and promote anti-tumor activity. MICA, a member of the MIC family, is widely expressed on many tumors, and it is the MICA/NKG2D interaction that is thought to stimulate the anti-tumor reactivity by T lymphocytes. Both MICA and MICB mRNA are widely expressed in normal tissues, with MICA being present in virtually every tissue except the nervous system, suggesting that MIC protein expression may only be one component of the anti-tumor activity of the immune system.
Background Reference 1:
Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol. 2006; 24:419-66.
Background Reference 2:
Bahram S, Bresnahan M, Geraghty DE, et al. A second lineage of mammalian major histocompatibility complex I genes. Proc. Natl. Acad. Sci. USA 1994; 91:6259-63.
Background Reference 3:
Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science 1999; 285:727-9.
Background Reference 4:
Maccalli C, Pende D, Castelli C, et al. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur. J. Immunol. 2003; 33:2033-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4261

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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