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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

MID1 Antibody

CATALOG NUMBER: 57-060

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the C-terminal region of human MID1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MID1
Additional Names:
E3 ubiquitin-protein ligase Midline-1, 632-, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF
Protein Accession Number:
O15344
Ncbi Gene Id Number:
4281
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thetripartite motif (TRIM) family, also known as the 'RING-Bbox-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIMmotif includes three zinc-binding domains, a RING, a B-box type 1and a B-box type 2, and a coiled-coil region. This protein formshomodimers which associate with microtubules in the cytoplasm. Theprotein is likely involved in the formation of multiproteinstructures acting as anchor points to microtubules. Mutations inthis gene have been associated with the X-linked form of Opitzsyndrome, which is characterized by midline abnormalities such ascleft lip, laryngeal cleft, heart defects, hypospadias, andagenesis of the corpus callosum. This gene was also the firstexample of a gene subject to X inactivation in human while escapingit in mouse. Multiple different transcript variants are generatedby alternate splicing; however, the full-length nature of some ofthe variants has not been determined.
Background Reference 1:
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Background Reference 2:
Treutlein, J., et al. Arch. Gen. Psychiatry 66(7):773-784(2009)
Background Reference 3:
Styrkarsdottir, U., et al. Nat. Genet. 41(1):15-17(2009)
Background Reference 4:
Scapoli, L., et al. Eur. J. Oral Sci. 116(6):507-511(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-060

List Size:
400 ul

List Price:

$495.00

Datasheet
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