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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1I with C-terminal MYC tag probed with 42-644 (1ug/ml) in Lane A and probed with
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1I with C-terminal MYC tag probed with 42-644 (1ug/ml) in Lane A and probed with

MID1IP1 Antibody

CATALOG NUMBER: 42-644

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HTVLSKLTRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing full-length Human MID1IP1 (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Specificity:
Reported variants represent identical protein: NP_001092261.1, NP_067065.1, NP_001092260.1
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MID1IP1
Additional Names:
MID1IP1, MID1 interacting protein 1 (gastrulation specific G12 homolog (zebrafish)), FLJ10386, G12-like, MIG12, STRAIT11499, THRSPL, MID1 interacting G12-like protein, MID1 interacting protein 1 (gastrulation specific G12-like)
Protein Accession Number:
NP_067065.1
Protein Gi Number:
10864051
Ncbi Gene Id Number:
58526

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-644

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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