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45-887 (0.1ug/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-887 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-887 (0.1ug/ml) staining of Mouse (A) and Rat (B) Heart, and Rat Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MID2 Antibody

CATALOG NUMBER: 45-887

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: QESPYVSGMKTCH
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognise both human isoforms of this protein.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MID2
Additional Names:
MID2, TRIM1, midline 2, FXY2, midin 2, tripartite motif protein 1, RP6-191P20.2, FLJ37715, FLJ41813, FXY2, RNF60,
Protein Accession Number:
NP_036348.2,NP_438112.2
Protein Gi Number:
6912504
Ncbi Gene Id Number:
11043
Background Reference 1:
Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-887

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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