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To detect hMidkine by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Midkine (XP-5230Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/ml overnight at 4C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/ml overnight at 4C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
To detect hMidkine by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Midkine (XP-5230Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.

Midkine Antibody

CATALOG NUMBER: XP-5230

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMidkine.
Sandwich:
To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Midkine (XP-5230Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.
Western Blot:
To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hMidkine specific antibody was purified by affinity chromatography employing immobilized hMidkine matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Midkine antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
MDK
Additional Names:
MK, ARAP, NEGF2, MK1, Midkine, Amphiregulin-associated protein, MK
Protein Accession Number:
P21741
Protein Gi Number:
127116
Ncbi Gene Id Number:
4192
User Note:
Centrifuge vial prior to opening.
Background:
Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5230

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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