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Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Mimitin Antibody

CATALOG NUMBER: 5445

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.
The immunogen is located within the last 50 amino acids of Mimitin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 17-007 - Raji Cell Slide
Purification:
Mimitin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Mimitin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFAF2
Additional Names:
Mimitin Antibody: MMTN, B17.2L, mimitin, NDUFA12L, Mimitin, mitochondrial, Myc-induced mitochondrial protein, MMTN
Protein Accession Number:
NP_777549
Protein Gi Number:
29789409
Ncbi Gene Id Number:
91942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
Background Reference 1:
Tsuneoka M, Teye K, Arima M, et al. A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma. J. Biol. Chem.2005; 280:19977-85.
Background Reference 2:
Wegrzyn P, Yarwood SJ, Fiegler N, et al. Mimitin - a novel cytokine-regulated mitochondrial protein. BMC Cell Biol.2009; 10:23.
Background Reference 3:
Ogilvie I, Kennaway NG, and Shoubridge EA. A molecular chaperone for mitochondrial complex I assembly is mutated in a progressive encephalopathy. J. Clin. Invest.2005; 115:2784-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5445

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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