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Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.
Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.
Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

MINA Antibody

CATALOG NUMBER: 5617

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (80%)
Immunogen:
MINA antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MINA.
The immunogen is located within amino acids 380 - 430 of MINA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
MINA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MINA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MINA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MINA
Additional Names:
MINA Antibody: ROX, MDIG, NO52, MINA53, Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 60S ribosomal protein L27a histidine hydroxylase, ROX
Protein Accession Number:
NP_694822
Protein Gi Number:
110227621
Ncbi Gene Id Number:
84864
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MINA Antibody: MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.
Background Reference 1:
Tsuneoka M, Kody Y, Soejima M, et al. A novel myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem.2002; 277:35450-9.
Background Reference 2:
Teye K, Tsuneoka M, Arima N, et al. Increased expression of a Myc target gene Mina53 in human colon cancer. Am. J. Pathol.2004; 164:205-16.
Background Reference 3:
Komiya K, Sueoka-Aragane N, Sato A, et al. Mina53, a novel c-Myc target gene, is frequently expressed in lung cancers and exerts oncogenic property in NIH/3T3 cells. J. Cancer Res. Clin. Oncol.2010; 136:465-73.
Background Reference 4:
Okamoto M, Van Stry M, Chung L, et al. Mina, an IL4 repressor, controls T helper type 2 bias. Nat. Immunol.2009; 10:872-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5617

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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