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To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Human MIP-1-alpha (XP-5232) as a capture antibody, allows the detection of at least 0.2 0.4 ng/well of recombinant Human MIP-1-alpha.
To detect Human MIP-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProScis Polyclonal Anti-Human MIP-1-alpha (XP-5232) as a capture antibody, allows the detection of at least 0.2 0.4 ng/well of recombinant Human MIP-1-alpha.

MIP-1a Antibody (biotin)

CATALOG NUMBER: XP-5232Bt

Clonality:
Polyclonal
Tested Applications:
E, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-1-alpha (human MIP-1-alpha).
Conjugate:
Biotin
Tested Applications:
E, WB
Application Note:
ELISA:

Sandwich:
To detect hMIP-1α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human MIP-1α (XP-5232) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α.

Western Blot:

To detect hMIP-1-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hMIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
Physical State:
Lyophilized
Storage Conditions:
MIP-1a antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL3
Additional Names:
MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha
Protein Accession Number:
P10147
Protein Gi Number:
127078
Ncbi Gene Id Number:
6348
User Note:
Centrifuge vial prior to opening.
Background:
MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5232Bt

List Size:
0.05 mg

List Price:

$335.00

Out of stock

Datasheet
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