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MIP-3 alpha Antibody [G3_4G10.3]

CATALOG NUMBER: 38-236

Clonality:
Monoclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
MIP-3 alpha antibody is produced in mice using highly pure (>98%) recombinant human MIP- 3alpha as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with our biotinylated antigen affinity purified anti-murine IL-10 (XP-5162Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MIP-3 alpha lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
CCL20
Additional Names:
Macrophage Inflammatory Protein 3 alpha, SCYA20, Small inducible cytokine A20, LARC, Liver and activation regulated chemokine, exodus-1, MIP3A, C-C motif chemokine 20, Beta-chemokine exodus-1, MIP-3-alpha, CKb4, ST38, MIP-3a
Protein Accession Number:
P78556
Protein Gi Number:
2829668
Ncbi Gene Id Number:
6364
User Note:
Centrifuge vial prior to opening.
Background:
Macrophage Inflammatory Protein 3 alpha is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. It inhibits proliferation of myeloid progenitors in colony formation assays. It binds to CCR6.

Was product number XP-5713-M

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-236

List Size:
0.5 mg

List Price:

$376.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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