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This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
This antibody stained PBMC. The primary antibody was incubated at 5.0 mg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.

MIP-3 beta Antibody [G5_4E11.3]

CATALOG NUMBER: 38-237

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
MIP-3-beta antibody is produced in BALB/c mice using highly pure (>98%) recombinant human MIP-3beta as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 14.0-16.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant rat IL-2 when used with our biotinylated antigen affinity purified anti-rat IL-2 (38-205) as the detection antibody at a concentration of at least 2.0-4.0 μg/mL.
Western Blot:
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MIP-3-beta lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
CCL23
Additional Names:
Macrophage Inflammatory Protein 3 beta, SCYA20, Small inducible cytokine A20, LARC, Liver and activation regulated chemokine, exodus-1, MIP3, MPIF1, SCYA23, C-C motif chemokine 23, CK-beta-8, CKB-8, CKb8, Ckb-8, MIP-3, MPIF-1, Ckb-8-1, hmrp-2a, CK-BETA-8
Protein Accession Number:
P55773
Protein Gi Number:
308153623
Ncbi Gene Id Number:
6368
User Note:
Centrifuge vial prior to opening.
Background:
Was product number XP-5714-M

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-237

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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