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To detect Human MIP-3-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIP-3-alpha (XP-5240Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-alpha.
To detect HumanMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect HumanMIP-3-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect Human MIP-3-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human MIP-3-alpha (XP-5240Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-alpha.

MIP-3a Antibody

CATALOG NUMBER: XP-5240

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3-alpha (human Macrophage Inflammatory Protein-3-alpha).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:To detect hMIP-3-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3-alpha.
Sandwich:
To detect hMIP-3α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-3α (XP-5240Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3α.
Western Blot:
To detect hMIP-3α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hMIP-3-alpha specific antibody was purified by affinity chromatography employing immobilized hMIP-3-alpha matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MIP-3a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL20
Additional Names:
CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, MIP-3-alpha, C-C motif chemokine 20, Beta-chemokine exodus-1
Protein Accession Number:
P78556
Protein Gi Number:
2829668
Ncbi Gene Id Number:
6364
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5240

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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