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To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5.
To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5.

MIP-5 (CCL15) Antibody (biotin)

CATALOG NUMBER: 38-338

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5.
Western Blot
To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
MIP-5 specific antibody was purified by affinity chromatography and then biotinylated
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CCL15
Additional Names:
Macrophage Inflammatory Protein-5, CCL15, HCC-2, Lkn-1
Protein Accession Number:
Q16663
Protein Gi Number:
Q16663.3
Ncbi Gene Id Number:
6359
User Note:
Centrifuge vial prior to opening.
Background:
MIP-5 is a CC chemokine that is expressed in the heart, skeletal muscle and adrenal gland. MIP-5 primarily signals through the CCR1 receptor, but has also been found to bind to CCR3. MIP-5 is chemotactic towards T cells and monocytes. Recombinant Human MIP-5 is a 10.1 kDa protein containing 92 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-338

List Size:
0.025 mg

List Price:

$391.00

Datasheet
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