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Western blot analysis of extracts of various cell lines, using MIP antibody (18-849) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MIP antibody (18-849) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MIP antibody (18-849) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MIP antibody (18-849) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MIP Antibody

CATALOG NUMBER: 18-849

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:100 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
U-251MG
Positive Control 3:
Mouse skeletal muscle
Positive Control 4:
Mouse heart
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 28kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MIP
Additional Names:
AQPCTRCT15, LIM126, MP26, MIP, lens fiber major intrinsic protein, aquaporin 0
Ncbi Gene Id Number:
4284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-849

List Size:
100 uL

List Price:

$436.00

Datasheet
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