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Western blot analysis of MIPU1 in human heart tissue lysate with MIPU1 antibody at 1 μg/mL.
Immunohistochemistry of MIPU1 in human brain tissue with MIPU1 antibody at 2.5 μg/mL.
Immunofluorescence of MIPU in human brain tissue with MIPU antibody at 20 μg/mL.
Western blot analysis of MIPU1 in human heart tissue lysate with MIPU1 antibody at 1 μg/mL.
Trial Size

MIPU1 Antibody

CATALOG NUMBER: 5219

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MIPU1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human MIPU1.
The immunogen is located within the first 50 amino acids of MIPU1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MIPU1 antibody can be used for detection of MIPU1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
MIPU1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MIPU1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MIPU1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZNF667
Additional Names:
MIPU1 Antibody: MIPU1, Zinc finger protein 667
Protein Accession Number:
NP_071386
Protein Gi Number:
38524600
Ncbi Gene Id Number:
63934
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MIPU1 Antibody: Mipu1, also known as zinc finger protein 667 or ZNF667, encodes a nuclear-localized protein containing 14 carboxy-terminal zinc finger motifs and an amino-terminal KRAB domain. This protein is highly expressed in heart and brain and is upregulated in rat heart after a transient ischemia-reperfusion procedure. Overexpression experiments suggest that Mipu1 suppresses the transcriptional activities of AP-1 and SRE in the MAPK signaling pathway and thus may play a role in the pathogenesis of cardiac and vascular disease. At least four isoforms of MIPU1 are known to exist.
Background Reference 1:
Jiang L, Tang D, Wang K, et al. Functional analysis of a novel KRAB/C2H2 zinc finger protein Mipu1. Biochem. Biophys. Res. Commun.2007; 356:829-35.
Background Reference 2:
Wang G, Zuo X, Jiang L, et al. Tissue expression and subcellular localization of Mipu1, a novel myocardial ischemia-related gene. Braz. J. Biol. Res.2009; epub.
Background Reference 3:
Yuan C, Zhang HL, Liu Y, et al. Cloning and characterization of a new gene Mipu1 up-regulated during myocardial ischemia-reperfusion. Prog. Biochem. Biophys.2004; 31:231-6.
Background Reference 4:
Wang G, Zuo X, Yuan C, et al. Mipu1, a novel rat zinc-finger protein, inhibits transcriptional activities of AP-1 and SRE in mitogen-activated protein kinase signaling pathway. Mol. Cell. Biochem.2009; 322:93-102.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5219

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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