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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

MITF Antibody

CATALOG NUMBER: 28-763

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat, Zebrafish
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MITF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MITF antibody can be used for detection of MITF by ELISA at 1:1562500. MITF antibody can be used for detection of MITF by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
47 kDa, 58 kDa, 46 kDa, 58 kDa, 56 kDa, 55 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MITF antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MITF
Additional Names:
MITF, MI, WS2, CMM8, WS2A, bHLHe32
Protein Accession Number:
NP_000239
Protein Gi Number:
4557755
Ncbi Gene Id Number:
4286
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background Reference 1:
Park, H.Y., et al., (2006) Biochem. J. 395 (3), 571-578.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-763

List Size:
100 ul

List Price:

$473.00

Datasheet
Shipping Info

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