Mitochondrial Antibody [MTL56]

Application Note:

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
IF: 0.5-1 ug/ml
Western blot: 1-2 ug/ml
Titering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.