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43-313 (0.5ug/ml) staining of MEF lysates (35ug protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with 43-313 (0.5ug/ml) in the left panel and with EB11930 (0.5ug/ml) in the right panel.
43-313 and EB11930 (1.5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and s
43-313 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
43-313 (0.5ug/ml) staining of MEF lysates (35ug protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.

MK2 / MAPKAPK2 Antibody

CATALOG NUMBER: 43-313

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, IP, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SRVLKEDKERWED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, IP, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MK2 / MAPKAPK2
Additional Names:
MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2
Protein Accession Number:
NP_004750.1,NP_116584.2
Protein Gi Number:
32481209
Ncbi Gene Id Number:
9261
Background Reference 1:
Yang Y, Xia F, Hermance N, Mabb A, Simonson S, Morrissey S, Gandhi P, Munson M, Miyamoto S, Kelliher MA.A cytosolic ATM/NEMO/RIP1 complex recruits TAK1 to mediate the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-313

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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