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Western blot analysis of extracts of Jurkat cells, using MKRN2 antibody (19-416).<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of Jurkat cells, using MKRN2 antibody (19-416).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MKRN2 Antibody [D9H10]

CATALOG NUMBER: 19-416

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic Peptide of human MKRN2
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:20 - 1:50
Flow: 1:20 - 1:50
Positive Control 1:
Jurkat
Predicted Molecular Weight:
Observed: 53kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MKRN2
Additional Names:
MKRN2, HSPC070, RNF62
Ncbi Gene Id Number:
23609
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-416

List Size:
100 uL

List Price:

$365.00

Out of stock

Datasheet
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