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Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.
Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
Trial Size

MKRN3 Antibody

CATALOG NUMBER: 8145

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MKRN3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MKRN3.
The immunogen is located within amino acids 400 - 450 of MKRN3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 55 kDa
Observed: 55 kDa
Purification:
MKRN3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MKRN3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MKRN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MKRN3
Additional Names:
Makorin ring finger 3, CPPB2, D15S9, RNF63, ZFP127, ZNF127
Protein Accession Number:
NP_005655
Protein Gi Number:
5032243
Ncbi Gene Id Number:
7681
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Makorin ring finger 3 (MKRN3) protein contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. The MKRN3 gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome (1), but a deletion of the gene does not (2). A deficiency of MKRN3 has been shown to cause central precocious puberty in humans (3).
Background Reference 1:
Jong MT, Gray TA, Ji Y, et al. A novel imprinted gene, encoding a RING zinc-finger protein, a overlapping antisense transcript in the Prader-Willi syndrome critical region. Hum. Mol. Genet. 1999; 8:783-93.
Background Reference 2:
Kanber D, Giltay J, Wieczorek D, et al. A paternal deletion of MKRN3, MAGEL2 and NDN does not result in Prader-Willi syndrome. Eur. J. Hum. Genet. 2009; 17:582-90.
Background Reference 3:
Abreu AP, Dauber A, Macedo DB, et al. Central precocious puberty caused by mutations in the imprinting gene MKRN3. N. Engl. J. Med. 2013; 368:2467-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8145

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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