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43-286 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-286 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-286 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

MLC1 Antibody

CATALOG NUMBER: 43-286

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SDSANILDEVPFPAR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 41.1kDa according to NP_055981.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (López-Hernández et al, Hum Mol Genet. 2011 Aug 15;20(16):3266-77.PMID:21624973). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5ug/ml.
Specificity:
Reported variants represent identical protein: NP_631941.1, NP_055981.1.
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MLC1
Additional Names:
MLC1, megalencephalic leukoencephalopathy with subcortical cysts 1, LVM, MLC, VL, membrane protein MLC1
Protein Accession Number:
NP_055981.1
Protein Gi Number:
14589896
Ncbi Gene Id Number:
23209
Background Reference 1:
López-Hernández T, Sirisi S, Capdevila-Nortes X, Montolio M, Fernández-Dueñas V, Scheper GC, van der Knaap MS, Casquero P, Ciruela F, Ferrer I, Nunes V, Estévez R.Molecular mechanisms of MLC1 and GLIALCAM mutations in megalencephalic leukoencephalopathy with subcortical cysts.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-286

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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