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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

MLH1 Antibody

CATALOG NUMBER: 55-988

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-480 amino acids from the Central region of human MLH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MLH1
Additional Names:
DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1, COCA2
Protein Accession Number:
P40692
Protein Gi Number:
730028
Ncbi Gene Id Number:
4292
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene was identified as a locus frequently mutated inhereditary nonpolyposis colon cancer (HNPCC). It is a human homologof the E. coli DNA mismatch repair gene mutL, consistent with thecharacteristic alterations in microsatellite sequences(RER+phenotype) found in HNPCC. Alternative splicing results inmultiple transcript variants encoding distinct isoforms. Additionaltranscript variants have been described, but their full-lengthnatures have not been determined.
Background Reference 1:
Ling, Z.Q., et al. Cancer Lett. 297(2):244-251(2010)
Background Reference 2:
Qi, Y., et al. J. Biol. Chem. 285(43):33010-33017(2010)
Background Reference 3:
Borras, E., et al. Cancer Res. 70(19):7379-7391(2010)
Background Reference 4:
Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-988

List Size:
400 ul

List Price:

$495.00

Datasheet
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