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Western blot analysis of MLIP in 293 cell lysate with MLIP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of MLIP in human heart tissue with MLIP antibody at 5 μg/mL.
Immunofluorescence of MLIP in human heart tissue with MLIP antibody at 20 μg/mL.
Western blot analysis of MLIP in 293 cell lysate with MLIP antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

MLIP Antibody

CATALOG NUMBER: 6719

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
MLIP antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human MLIP.
The immunogen is located within the last 50 amino acids of MLIP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MLIP antibody can be used for detection of MLIP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of MLIP are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
MLIP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MLIP antibody can be stored at 4°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MLIP
Additional Names:
MLIP Antibody: C6orf142, C6orf142, Muscular LMNA-interacting protein, Muscular-enriched A-type laminin-interacting protein
Protein Accession Number:
NP_612636
Protein Gi Number:
154426278
Ncbi Gene Id Number:
90523
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MLIP Antibody: The muscle-enriched A-type lamin-interacting protein (MLIP) is ubiquitously expressed, although most predominantly in heart, skeletal and smooth muscle, and interacts directly and co-localizes with lamin A and C in the nuclear envelope. MLIP also co-localizes with promyelocytic leukemia (PML) bodies within the nucleus. Decreasing lamin A/C expression with siRNA results in the upregulation and mislocalization of MLIP, suggesting that MLIP localization to the nuclear envelope is dependent on the lamin proteins. It has been suggested that MLIP may function as a tethering protein linking LMNA to factors within the nuclear envelope of within PML bodies
Background Reference 1:
Ahmady E, Deeke SA, Rabaa S, et al. Identification of a novel muscle A-type lamin-interacting protein (MLIP). J. Biol. Chem. 2011; 286:19702-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6719

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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